"The prevalence among women aged 25 and above has stabilised at high and unacceptable levels," Motsoaledi said in Pretoria, releasing the 2008 results of a survey of antenatal HIV.
He said the report was a useful tool to observe trends and increase commitment to the implementation of government policies, as well as to provide feedback to health workers.
In 2007 antenatal HIV prevalence was 29.4 percent and in 2008 29.3 percent.
About 33,927 women aged between 15 and 49 who attended antenatal clinic in public health sectors participated in the survey.
HIV prevalence in the 15 to 24 year age group declined 0.4 percent from 22.1 percent in 2007 to 21.7 in 2008.
The highest prevalence was in the 30 to 34 age group, at 39.6 percent in 2007 and 40.4 percent in 2008.
According to the report KwaZulu-Natal has the highest prevalence followed by Mpumalanga, Free State and North West.
Gauteng, Limpopo and Eastern Cape have prevalence of between 20 and 30 percent while the Northern Cape and Western Cape have lowest prevalence of below 20 percent.
Motsoaledi said there was a need to work with academics and researchers to find new ways to respond to the problem.
"What cannot be contested is that the burden of HIV and AIDS is now weighing heavily on the shoulder of our country," he said.
He said the findings of the report confirmed earlier reports of the general population survey done by the Human Science Research Council and Medical Research Council that the battle seemed to be lost in KwaZulu-Natal and Mpumalanga.
"We seem to be loosing the battle but not yet the war," he said.
The report stated there was a need to report HIV prevalence distribution by geographic area (rural, semi rural and urban) as this would assist in interventions.
Motsoaledi said he was optimistic that the next survey would be "more encouraging".
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