The relocation of some of the Kruger National Park's white rhino began on Wednesday, SA National Parks said.
This followed a rhino management strategy adopted by Cabinet in August to curb poaching.
"Relocation is the core of our approach, not only to combat poaching, but to ensure the continued growth of the rhino population," SANParks chairman Kuseni Dlamini said in a statement.
"We aim to restimulate growth in large protected areas while creating new rhino strongholds. In this way, we will be able to offset the effects of poaching in the short to medium-term, while expanding rhino range and improving the overall population size."
Auctioning rhino in line with the management strategy would take place later in the year, he said.