The Scots were due to face a Zimbabwe XI in October but they revealed on Tuesday that their players would not travel as a result of government advice about the volatile political climate in the African nation.
Following advice from security and government official, Cricket Scotland had agreed to move the match to Zimbabwe after it was orginally scheduled for a neutral African country.
But Scottish officials have now informed the International Cricket Council (ICC) of their decision to pull out of the trip.
Cricket Scotland chief executive Roddy Smith said: "It is hugely unfortunate that Cricket Scotland has been put in an impossible no-win situation in regard to the potential tour to Zimbabwe.
"Our board has taken cognisance of all the advice and recommendations from Government and the ICC, and can only take what we believe is the correct course of action.
"Both the UK and Scottish Governments were clear in their advice to us.
"Although accepting that this decision will not be welcomed by some key partners, we felt that as a responsible governing body we could not, and would not, contravene the direct and unequivocal advice from Government."
The organisation published advice from the UK Government which stated that there "has not yet been sufficient progress in Zimbabwe on the fundamental issues of political reform and of re-establishing the rule of law to justify sports tours by British teams and the positive signal that would send".
Just R20 for the first month. Support independent journalism by subscribing to our digital news package.