The award-winning artist, who is credited with helping to fund vaccine research and the trials rollout after donating $1m (about R14.8m) to Vanderbilt University Medical Center, received the jab at the university in Nashville, Tennessee on Tuesday.
In a video posted by the 75-year-old icon, Parton adapts the words of her smash 1974 hit Jolene, encouraging others to “get out there and get your shot”.
