Of all the animals on the list, it is the buffalo which poses the biggest risk to humans - at least during the day.
Notoriously grumpy and frighteningly fast on its hooves, the Cape buffalo has a markedly different personality to the docile water buffalo you may see being ridden by children in lovely photographs in travel stories about of Southeast Asia.
Andrew Zaloumis, iSimangaliso park chief executive, learned this first hand last year when he and his wife, Tracey, were trampled and gored by a buffalo while walking on the eastern shores of Lake St Lucia, which lies at the heart of the park.
"It was my fault," he said. "I was walking along, with my head in the office and not concentrating about where I was, when we stumbled across some buffalo in a thicket." Zaloumis had barely a second to think before the buffalo charged the couple, leaving them both with broken bones and deep lacerations.
It is no surprise, then, that the guides take the briefing seriously.
Lead guide Mandla Buthelezi says simply, "if I tell you to lie flat, lie flat."
LISTEN: In this podcast, Buthelezi talks to Sunday Times travel editor Paul Ash about buffaloes, lions and what an angry leopard sounds like.